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KMID : 0380020130280020123
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 2 p.123 ~ p.130
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Effect of Pine needle Ethanol Extracts on the Inhibitory Activity of Atopic Dermatitis
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Jeong Da-Hyun
Kim Koth-Bong-Woo-Ri
Jung Seul-A
Kim Hyun-Jee
Kang Bo-Kyeong
Bark Si-Woo
Kim Tae-Wan
Ahn Dong-Hyun
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Abstract
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The aim of this study was to examine inhibitory effects of pine needle ethanol extracts (PNEE) on atopic dermatitis (AD). To determine inflammatory activity PNEE was added to LPS-induced murine peritoneal macrophages for an in-vitro test. In addition, anti-AD test was carried out by spreading PNEE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)- induced BALB/c mice. It was confirmed that the nitric oxide (NO) secretion was suppressed when 1~50 μg/mL of PNEE were added to LPS-induced murine peritoneal macrophages. Moreover, levels of TNF-α, IL-6, and IL-1β, were decreased. For the anti-AD test, PNEE alleviated symptoms of the erythema in DNCB-induced mice. Furthermore, the IFN-γ secretion of the group treated with PNEE was increased in splenocytes from DNCB-induced mice compared to the positive control, while IL-4 secretion diminished. Through these results, we can conclude that PNEE can inhibit AD by modulating the IFN-γ, IL-4 cytokines production and inhibiting inflammation.
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KEYWORD
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Pine needle, Anti-atopy, Anti-inflammation
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